Alkaline Phosphatase/ALPL Antibody (388828)

Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 388828
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Alkaline Phosphatase/ALPL Antibody (388828) Summary

• Immunogen: E. coli-derived recombinant human Alkaline Phosphatase/ALPL
  Leu18-Ser502
  Accession # P05186
• Specificity: Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100% cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP or rhAlkaline Phosphatase/ALPI is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ALPL
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (388828)

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase/ALPL Antibody (MAB29091)(4)

Publications using MAB29091

• R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis Nat Commun, 2020;11(1):460. 2020 [PMID: 31974363] (IHC, Mouse)
• Onishi K, Tonge P, Nagy A, Zandstra P Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency. Stem Cell Reports, 2014;3(1):156-68. 2014 [PMID: 25068129] (ICC, Mouse)
• Foster BL, Nagatomo KJ, Nociti FH, Fong H, Dunn D, Tran AB, Wang W, Narisawa S, Millan JL, Somerman MJ Central role of pyrophosphate in acellular cementum formation. PLoS ONE, 2012;7(6):e38393. 2012 [PMID: 22675556] (IHC, Mouse)
• Yadav MC, de Oliveira RC, Foster BL, Fong H, Cory E, Narisawa S, Sah RL, Somerman M, Whyte MP, Millan JL Enzyme replacement prevents enamel defects in hypophosphatasia mice. J. Bone Miner. Res., 2012;27(8):1722-34. 2012 [PMID: 22461224] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ALPL
• Uniprot:
  • P05186()