Alkaline Phosphatase/ALPL Antibody (388828) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 388828
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Alkaline Phosphatase/ALPL Antibody (388828) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Alkaline Phosphatase/ALPL
  Leu18-Ser502
  Accession # P05186
• Specificity: Detects human Alkaline Phosphatase/ALPL in direct ELISAs. In direct ELISAs, 80-100% cross-reactivity with recombinant mouse Alkaline Phosphatase/ALPL is observed and no cross-reactivity with recombinant human (rh) Alkaline Phosphatase/ALPP or rhAlkaline P
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (388828) [Alexa Fluor® 405]

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.