Alix Antibody (S09-4B4)

Western Blot: Alix Antibody (S09-4B4) [NBP3-19564] - Western blot detection of Alix in HT1080 cell lysates using NBP3-19564 (1:1000 diluted). Predicted band size: 96KDa. Observed band size: 96KDa.

• Reactivity: Hu
• Applications: WB
• Clone: S09-4B4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Alix Antibody (S09-4B4) Summary

• Description: Novus Biologicals Rabbit Alix Antibody (S09-4B4) (NBP3-19564) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human Alix (Uniprot # Q8WUM4)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDCD6IP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/2000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Alix Antibody (S09-4B4)

• AIP1DRIP4
• ALG-2 interacting protein 1
• ALG-2 interacting protein X
• ALG-2-interacting protein 1
• alinx
• Alix
• apoptosis-linked gene 2-interacting protein X
• dopamine receptor interacting protein 4
• HP95
• KIAA1375
• MGC17003
• PDCD6-interacting protein
• programmed cell death 6 interacting protein
• programmed cell death 6-interacting protein

Background

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD6IP