Alix Antibody (3A9)

Images

 
Western Blot: Alix Antibody (3A9) [NB100-65678] - Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (STAR13B)

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Alix Antibody (3A9) Summary

Immunogen
Alix fusion protein
Specificity
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDCD6IP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For IHC - This product does not require protein digestion pre-treatment of paraffin sections.
Publications
Read Publication using NB100-65678.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Alix Antibody (3A9)

  • AIP1DRIP4
  • ALG-2 interacting protein 1
  • ALG-2 interacting protein X
  • ALG-2-interacting protein 1
  • alinx
  • Alix
  • apoptosis-linked gene 2-interacting protein X
  • dopamine receptor interacting protein 4
  • HP95
  • KIAA1375
  • MGC17003
  • PDCD6-interacting protein
  • programmed cell death 6 interacting protein
  • programmed cell death 6-interacting protein

Background

ALG2 interacting protein X is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. It may play a role in the regulation of both apoptosis and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Alix Antibody (NB100-65678)(1)

We have publications tested in 1 confirmed species: Mouse.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Alix Antibody (NB100-65678) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65678AF405
Alexa Fluor 488 Labeled NB100-65678AF488
Alexa Fluor 647 Labeled NB100-65678AF647
Alexa Fluor 700 Labeled NB100-65678AF700
DyLight 350 Labeled NB100-65678UV
DyLight 405 Labeled NB100-65678V
DyLight 488 Labeled NB100-65678G
DyLight 680 Labeled NB100-65678FR
DyLight 755 Labeled NB100-65678IR

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Blogs on Alix.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

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Bioinformatics

Gene Symbol PDCD6IP
Entrez
Uniprot