Home » Alix » Alix Antibodies » Alix Antibody (3A9) [HRP]

Alix Antibody (3A9) [HRP]

 Submit a Review

Images

 
There are currently no images for Alix Antibody (NB100-65678H).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-65678AF405
Alexa Fluor 488
NB100-65678AF488
Alexa Fluor 647
NB100-65678AF647
Alexa Fluor 700
NB100-65678AF700
DyLight 350
NB100-65678UV
DyLight 405
NB100-65678V
DyLight 488
NB100-65678G
DyLight 680
NB100-65678FR
DyLight 755
NB100-65678IR
Janelia Fluor 549
NB100-65678JF549
Janelia Fluor 646
NB100-65678JF646
Unconjugated
NB100-65678
100% Guarantee on every product

Alix Antibody (3A9) [HRP] Summary

Immunogen
Alix fusion protein
Specificity
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDCD6IP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alix Antibody (3A9) [HRP]

  • AIP1DRIP4
  • ALG-2 interacting protein 1
  • ALG-2 interacting protein X
  • ALG-2-interacting protein 1
  • alinx
  • Alix
  • apoptosis-linked gene 2-interacting protein X
  • dopamine receptor interacting protein 4
  • HP95
  • KIAA1375
  • MGC17003
  • PDCD6-interacting protein
  • programmed cell death 6 interacting protein
  • programmed cell death 6-interacting protein

Background

ALG2 interacting protein X is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. It may play a role in the regulation of both apoptosis and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

PDCD6 Antibody
NBP2-19741
Western Blot: PDCD6 Antibody [NBP2-19741] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/Immunofluorescence: PDCD6 Antibody [NBP2-19741] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PDCD6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: PDCD6 Antibody [NBP2-19741] - Paraffin-embedded human colon carcinoma. PDCD6 antibody diluted at 1:500.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TSG101 Antibody
(4A10)
NB200-112
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Western Blot: TSG101 Antibody (4A10) [Biotin] [NB200-112B] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: C2C12 10% SDS PAGE diluted at 1:500 Image using the Biotin form of this antibody.
Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Paraffin-embedded Colon carcinoma, using antibody at 1:100 dilution.
Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.
(2 Reviews)
(67 Publications)
Species: Hu, Mu, Rt, Ha, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KO
CHMP4A Knockout
HeLa Cell Lysate
NBP2-65248
CHMP4A Knockout HeLa Cell Lysate
Species: Hu
CHMP4B Antibody
NBP1-91782
Western Blot: CHMP4B Antibody [NBP1-91782] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human cerebral cortex shows high expression.
Species: Hu
Applications: WB, IHC, IHC-P
VPS4A Antibody
NBP1-54618
Western Blot: VPS4A Antibody [NBP1-54618] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Western Blot: VPS4A Antibody [NBP1-54618] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle
Species: Hu
Applications: WB, IHC
CIN85/SH3KBP1
Antibody
NBP1-85614
Western Blot: CIN85/SH3KBP1 Antibody [NBP1-85614] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: CIN85/SH3KBP1 Antibody [NBP1-85614] - Staining of human skeletal muscle shows low expression as expected.
Western Blot: CIN85/SH3KBP1 Antibody [NBP1-85614] - Analysis in human cell line THP-1.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
MAGI2 Antibody
AF7117
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAGI2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MAGI2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons and neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MAGI2 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu, Mu
Applications: WB, IHC, ICC
Destrin Antibody
(OTI2F7)
NBP2-00527
Western Blot: Destrin Antibody (2F7) [NBP2-00527] Analysis of extracts (35ug) from 9 different cell lines by using anti-Destrin monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Destrin Antibody (2F7) [NBP2-00527] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Destrin.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human prostate tissue using anti-Destrin mouse monoclonal antibody.
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(19 Reviews)
(511 Publications)
Species: Hu
EN-RAGE/S100A12
Antibody [Unconjugate
AF1052
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Reviews)
(2 Publications)
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
Exosome Component
9 Antibody
NBP1-71702
Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.
Immunocytochemistry/Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.
(2 Reviews)
(3 Publications)
Species: Hu, Mu, Ch
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
WDR1 Antibody
NBP2-32665
Western Blot: WDR1 Antibody [NBP2-32665] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: WDR1 Antibody [NBP2-32665] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: WDR1 Antibody [NBP2-32665] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Gelsolin/GSN
Antibody
NBP1-33177
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.
Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
VPS4B Antibody
NBP2-20881
Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.
Immunocytochemistry/Immunofluorescence: VPS4B Antibody [NBP2-20881] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Paraffin-embedded colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Glutathione S
Transferase kappa 1 A ...
NBP1-89727
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining in human small intestine and pancreas tissues using anti-GSTK1 antibody. Corresponding GSTK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
SLCO6A1 Antibody
NBP2-49118
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
PTPN23 Antibody
NBP1-84158
Immunocytochemistry/Immunofluorescence: PTPN23 Antibody [NBP1-84158] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
Immunohistochemistry-Paraffin: PTPN23 Antibody [NBP1-84158] - Staining of human appendix shows cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Alix Antibody
(3A9) [HRP]
NB100-65678H
Alix Antibody (3A9) [HRP]
Species: Hu, Mu, Rt, Xp
Applications: WB, ELISA, IHC, IHC-P

Publications for Alix Antibody (NB100-65678H) (0)

There are no publications for Alix Antibody (NB100-65678H).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Alix Antibody (NB100-65678H) (0)

There are no reviews for Alix Antibody (NB100-65678H). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Alix Antibody (NB100-65678H) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Alix Antibody (NB100-65678H)

Discover related pathways, diseases and genes to Alix Antibody (NB100-65678H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Alix Antibody (NB100-65678H)

Discover more about diseases related to Alix Antibody (NB100-65678H).
 

Pathways for Alix Antibody (NB100-65678H)

View related products by pathway.

PTMs for Alix Antibody (NB100-65678H)

Learn more about PTMs related to Alix Antibody (NB100-65678H).

Blogs on Alix.

Check out the latest blog posts on Alix.
Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

Customers Who Bought This Also Bought

Mouse IgG1 Isotype Control (MG1) [HRP]
NBP1-97074
Alix Recombinant Protein Antigen
NBP1-90201PEP
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
TNF-alpha [Unconjugated]
210-TA-005
Western Blot: Alix Overexpression Lysate (Adult Normal) [NBP2-10343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alix.
Alix Overexpression Lysate (Native)
NBP2-10343

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Alix Antibody (3A9) [HRP] and receive a gift card or discount.


Bioinformatics

Gene Symbol PDCD6IP
Entrez
Uniprot