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Alix Antibody (3A9) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-65678AF405
Alexa Fluor 488
NB100-65678AF488
Alexa Fluor 647
NB100-65678AF647
Alexa Fluor 700
NB100-65678AF700
DyLight 350
NB100-65678UV
DyLight 405
NB100-65678V
DyLight 488
NB100-65678G
DyLight 680
NB100-65678FR
DyLight 755
NB100-65678IR
Janelia Fluor 549
NB100-65678JF549
Janelia Fluor 646
NB100-65678JF646
Unconjugated
NB100-65678
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Alix Antibody (3A9) [HRP] Summary

Immunogen
Alix fusion protein
Specificity
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDCD6IP
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alix Antibody (3A9) [HRP]

  • AIP1DRIP4
  • ALG-2 interacting protein 1
  • ALG-2 interacting protein X
  • ALG-2-interacting protein 1
  • alinx
  • ALIX
  • apoptosis-linked gene 2-interacting protein X
  • dopamine receptor interacting protein 4
  • HP95
  • KIAA1375
  • MGC17003
  • PDCD6-interacting protein
  • programmed cell death 6 interacting protein
  • programmed cell death 6-interacting protein

Background

ALG2 interacting protein X is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. It may play a role in the regulation of both apoptosis and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: PDCD6 Antibody [NBP2-19741] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PDCD6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
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Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Paraffin-embedded Colon carcinoma, using antibody at 1:100 dilution.
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on HeLa cells with TSG101 Antibody (4A10) NB200-112F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
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NBP2-65248
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Western Blot: CHMP4B Antibody [NBP1-91782] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human cerebral cortex shows high expression.
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NBP1-54618
Western Blot: VPS4A Antibody [NBP1-54618] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Western Blot: VPS4A Antibody [NBP1-54618] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle
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NBP1-85614
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Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
MAGI2 Antibody
AF7117
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TNF-alpha
[Unconjugated]
210-TA
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AF1052
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Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.
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Alix Antibody
(3A9) [HRP]
NB100-65678H
Alix Antibody (3A9) [HRP]
Species: Hu, Mu, Rt, Xp
Applications: WB, ELISA, IHC, IHC-P

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Bioinformatics

Gene Symbol PDCD6IP
Entrez
Uniprot