ALG2 Antibody (OTI3C2)

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ALG2 monoclonal antibody.

Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734] - Staining of paraffin-embedded Human prostate tissue using anti-ALG2 mouse monoclonal antibody.

Western Blot: ALG2 Antibody (OTI3C2) [NBP2-01734] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2.

Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALG2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: ALG2 Antibody (OTI3C2) [NBP2-01734] - Staining of paraffin-embedded Human endometrium tissue using anti-ALG2 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI3C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.82 mg/ml

ALG2 Antibody (OTI3C2) Summary

• Immunogen: Full length human recombinant protein of human ALG2(NP_149078) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 46.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.82 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALG2 Antibody (OTI3C2)

• alpha-1,3-mannosyltransferase ALG2
• alpha-1,3-mannosyltransferase)
• asparagine-linked glycosylation 2 homolog (S. cerevisiae
• asparagine-linked glycosylation 2 homolog (yeast
• asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S.cerevisiae)
• Asparagine-linked glycosylation protein 2 homolog
• CDGIi
• EC 2.4.1
• EC 2.4.1.-
• FLJ14511
• GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
• hALPG2
• homolog of yeast ALG2
• NET38

Background

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALG2
• Entrez:
  • 85365(Human)