Aldo-keto Reductase 1C4/AKR1C4 Antibody

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C4/AKR1C4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6957) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C4/AKR1C4 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Aldo-keto Reductase 1C4/AKR1C4 Antibody Summary

• Immunogen: E. coli-derived recombinant human Aldo-keto Reductase 1C4/AKR1C4
  Met1-Tyr323
  Accession # NP_001809
• Specificity: Detects human Aldo-keto Reductase 1C4/AKR1C4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AKR-1C1 and rhAKR-1C3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AKR1C4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldo-keto Reductase 1C4/AKR1C4 Antibody

• 3-alpha-HSD
• AKR1C4
• Aldo-keto Reductase 1C4
• aldo-keto reductase family 1, member C4
• AldoketoReductase 1C4
• C11
• CDR
• CHDR
• DD4
• DD-4
• HAKRA

Background

AKR1C4 (Aldo-Keto Reductase family 1 member C4; also 3-alpha HSD1/3-alpha HSD type 1, Chlordecone/kepone reductase/CDR and DD4) is a monomeric, 36-38 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. AKRs catalyse the reduction of aldehydes and ketones into alcohols through a NADPH-dependent process. The resulting alcohols become the target of subsequent conjugation reactions. AKR1C4 in particular is noted for its action on dihydroxytestosterone, converting it to a less active 3 alpha -diol. In addition, it also converts progesterone into 20 alpha -hydroxyprogesterone, a molecule that both induces GnRH release, and blocks the generation of (deoxy)cortisol from 17 alpha -hydroxyprogesterone. Finally, AKR1C4 likely is involved in the tetoxification of xenobiotics and drugs. AKR1C4 expression is essentially limited to hepatocytes and likely breast epithelium. Human AKR1C4 is 323 amino acids (aa) in length. Full-length human AKR1C4 shares 83% and 76% aa sequence identity with human AKR1C1 and mouse AKR1C4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C4
• Uniprot:
  • NP_001809()