Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate

Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate [NBP2-07959] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1C3.

• Reactivity: Hu
• Applications: WB

Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate Summary

• Description:

  Aldo-keto Reductase 1C3/AKR1C3 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC200210
  
  Accession#: NM_003739
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: AKR1C3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 36.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate

• 17-beta-hydroxysteroid dehydrogenase type 5
• 3-alpha-HSD type 2
• 3-alpha-hydroxysteroid dehydrogenase type 2
• AKR1C3
• Aldo-keto Reductase 1C3
• aldo-keto reductase family 1 member C3
• aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• AldoketoReductase 1C3
• Chlordecone reductase homolog HAKRb
• DD3
• DD-3
• DD317-beta-HSD 5
• DDH1
• DDX
• Dihydrodiol dehydrogenase 3
• Dihydrodiol dehydrogenase type I
• dihydrodiol dehydrogenase X
• EC 1.1.1
• EC 1.1.1.112,3-alpha-HSD type II, brain
• EC 1.1.1.188
• EC 1.1.1.213
• EC 1.1.1.63
• EC 1.1.1.64
• EC 1.3.1.20
• HA1753
• HAKRB
• HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
• HAKRe
• HSD17B5
• hydroxysteroid (17-beta) dehydrogenase 5
• Indanol dehydrogenase
• KIAA0119hluPGFS
• PGFS
• Prostaglandin F synthase
• Testosterone 17-beta-dehydrogenase 5
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

AKR1C3 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C3