Aldo-keto Reductase 1B10/AKR1B10 Antibody

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Aldo-keto Reductase 1B10/AKR1B10 Antibody Summary

• Immunogen: Peptide with sequence C-QSSHLEDYPFDAE corresponding to C-Terminus according to NP_064695.2.
• Epitope: C-QSSHLEDYPFDAE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKR1B10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in lysates of HEK293, A549 and HepG2 (calculated MW of 36.0 kDa band according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Aldo-keto Reductase 1B10/AKR1B10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aldo-keto Reductase 1B10/AKR1B10 Antibody

• AKR1B10
• AKR1B11
• AKR1B12
• Aldo-keto Reductase 1B10
• aldo-keto reductase family 1 member B10
• aldo-keto reductase family 1, member B10 (aldose reductase)
• aldo-keto reductase family 1, member B11 (aldose reductase-like)
• AldoketoReductase 1B10
• aldose reductase-like 1
• aldose reductase-like peptide
• Aldose reductase-like
• Aldose reductase-related protein
• ALDRLn
• ARL1
• ARL-1SI reductase
• ARP
• EC 1.1.1
• EC 1.1.1.-
• EC 1.1.1.21
• hARP
• HIS
• HSI
• MGC14103
• Small intestine reductase

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)(1)

Publication using NB100-93370

• Tammali R, Ramana KV, Singhal SS et al. Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. Cancer Res 2006-10-01 [PMID: 17018629]

Bioinformatics

• Gene Symbol: AKR1B10
• Entrez:
  • 57016(Human)
• Uniprot:
  • NP_064695.2(Human)