Home » Aldo-keto Reductase 1B10/AKR1B10 » Aldo-keto Reductase 1B10/AKR1B10 Antibodies » Aldo-keto Reductase 1B10/AKR1B10 Antibody

Aldo-keto Reductase 1B10/AKR1B10 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the ...read more
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

100% Guarantee on every product

Aldo-keto Reductase 1B10/AKR1B10 Antibody Summary

Immunogen
Peptide with sequence C-QSSHLEDYPFDAE corresponding to C-Terminus according to NP_064695.2.
Epitope
C-QSSHLEDYPFDAE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AKR1B10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in lysates of HEK293, A549 and HepG2 (calculated MW of 36.0 kDa band according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
Control
Aldo-keto Reductase 1B10/AKR1B10 Overexpression Lysate
Publications
Read Publication using NB100-93370.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aldo-keto Reductase 1B10/AKR1B10 Antibody

  • AKR1B10
  • AKR1B11
  • AKR1B12
  • Aldo-keto Reductase 1B10
  • aldo-keto reductase family 1 member B10
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • aldo-keto reductase family 1, member B11 (aldose reductase-like)
  • AldoketoReductase 1B10
  • aldose reductase-like 1
  • aldose reductase-like peptide
  • Aldose reductase-like
  • Aldose reductase-related protein
  • ALDRLn
  • ARL1
  • ARL-1SI reductase
  • ARP
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.21
  • hARP
  • HIS
  • HSI
  • MGC14103
  • Small intestine reductase

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ACTR2 Antibody
(1B10-C4)
H00010097-M01
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Western Blot analysis of ACTR2 expression in A-431 ( Cat # L015V1 ).
Immunohistochemistry-Paraffin: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Analysis of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Sandwich ELISA: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Detection limit for recombinant GST tagged ACTR2 is approximately 0.03ng/ml as a capture antibody.
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
AKR1A1 Antibody
(OTI6E3)
NBP2-02164
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
alcohol
dehydrogenase 4 Antib
NBP1-90233
Immunohistochemistry-Paraffin: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 4 Antibody [NBP1-90233] - Staining in human liver and pancreas tissues using anti-ADH4 antibody. Corresponding ADH4 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
alcohol
dehydrogenase 1C Anti
NBP2-92824
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human esophagus using alcohol dehydrogenase 1C .
Western Blot: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Analysis of extracts of various cell lines, using alcohol dehydrogenase 1C .Exposure time: 10s.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human stomach using alcohol dehydrogenase 1C .
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
alcohol
dehydrogenase 1B Anti ...
NBP2-00649
Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Species: Ca, Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
alcohol
dehydrogenase Antibod
NB300-956
Western Blot: alcohol dehydrogenase Antibody [NB300-956] - Analysis of pan alcohol dehydrogenase in Human Liver lysate
Western Blot: alcohol dehydrogenase Antibody [NB300-956] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
alcohol
dehydrogenase 6 Antib
NBP2-62658
Immunohistochemistry-Paraffin: alcohol dehydrogenase 6 Antibody [NBP2-62658] - Analysis in human liver and pancreas tissues using Anti-ADH6 antibody. Corresponding ADH6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 6 Antibody [NBP2-62658] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 6 Antibody [NBP2-62658] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P, WB
ACTR3 Antibody
(8K4F2)
NBP3-16424
Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry of paraffin-embedded rat kidney using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry of paraffin-embedded human appendix using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ACTR3 Antibody (8K4F2) [NBP3-16424] - Immunohistochemistry of paraffin-embedded mouse spleen using ACTR3 Rabbit mAb (NBP3-16424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AKR1B1 Antibody
NBP1-89146
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Analysis in human adrenal gland and liver tissues. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AICDA Antibody
NBP1-30955
Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Angiopoietin-like
Protein 2/ANGPTL2 Ant ...
AF1444
Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Publications)
Species: Mu
Applications: IHC, WB
WASP Antibody
NB300-996
Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Pleiotrophin/PTN
Antibody [Unconjugate ...
AF-252-PB
Pleiotrophin/PTN was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Pleiotrophin/PTN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-252-PB) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(12 Publications)
Species: Hu
Applications: IHC, WB
N-WASP Antibody
NBP1-82512
Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP1-82512] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.
Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
Catalase Antibody
[Unconjugated]
AF3398
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, andRat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. 
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(3 Reviews)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
(1 Review)
(46 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
ARFRP1 Antibody
NBP2-14306
Western Blot: ARFRP1 Antibody [NBP2-14306] - Analysis in control (vector only transfected HEK293T lysate) and ARFRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ARFRP1 Antibody [NBP2-14306] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P, WB
APOBEC3A Antibody
NBP2-82648
Western Blot: APOBEC3A Antibody [NBP2-82648] - Host: Rabbit. Target Name: ABC3A. Sample Type: Fetal Kidney lysates. Antibody Dilution: 1ug/ml
Species: Hu
Applications: WB
Cortactin Antibody
(SC61-08)
NBP2-67632
Flow Cytometry: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Aldo-keto
Reductase 1B10/AKR1B1 ...
NB100-93370
Aldo-keto Reductase 1B10/AKR1B10 Antibody
Species: Hu
Applications: WB, PEP-ELISA

Publications for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)(1)


Reviews for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370) (0)

There are no reviews for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Aldo-keto Reductase 1B10/AKR1B10 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Additional Aldo-keto Reductase 1B10/AKR1B10 Products

Bioinformatics Tool for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)

Discover related pathways, diseases and genes to Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)

Discover more about diseases related to Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370).
 

Pathways for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)

View related products by pathway.

PTMs for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)

Learn more about PTMs related to Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370).
 

Research Areas for Aldo-keto Reductase 1B10/AKR1B10 Antibody (NB100-93370)

Find related products by research area.

Blogs on Aldo-keto Reductase 1B10/AKR1B10

There are no specific blogs for Aldo-keto Reductase 1B10/AKR1B10, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Aldo-keto Reductase 1B10/AKR1B10 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol AKR1B10
Entrez
Uniprot