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Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (316) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
316
Clonality
Monoclonal
Host
Rabbit
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90120AF350
Alexa Fluor 405
NBP2-90120AF405
Alexa Fluor 488
NBP2-90120AF488
Alexa Fluor 532
NBP2-90120AF532
Alexa Fluor 594
NBP2-90120AF594
Alexa Fluor 647
NBP2-90120AF647
Alexa Fluor 700
NBP2-90120AF700
Alexa Fluor 750
NBP2-90120AF750
Biotin
NBP2-90120B
DyLight 350
NBP2-90120UV
DyLight 405
NBP2-90120V
DyLight 550
NBP2-90120R
DyLight 594
NBP2-90120DL594
DyLight 650
NBP2-90120C
DyLight 680
NBP2-90120FR
DyLight 755
NBP2-90120IR
FITC
NBP2-90120F
HRP
NBP2-90120H
Unconjugated
NBP2-90120-100ul
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Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (316) [DyLight 488] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 (AAH04370.1; Met1-His453).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ALDH3A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (316) [DyLight 488]

  • aldehyde dehydrogenase 3 family, member A1
  • Aldehyde dehydrogenase 3
  • Aldehyde Dehydrogenase 3A1
  • Aldehyde Dehydrogenase 3-A1
  • Aldehyde dehydrogenase family 3 member A1
  • aldehyde dehydrogenase isozyme 3
  • aldehyde dehydrogenase type III
  • ALDH3A1
  • ALDH3aldehyde dehydrogenase, dimeric NADP-preferring
  • ALDHIII
  • EC 1.2.1
  • EC 1.2.1.5
  • MGC10406
  • stomach aldehyde dehydrogenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Aldehyde Dehydrogenase 1‑A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IP, ICC
ALDH2 Antibody
(4G6A3)
NBP2-37397
Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.
Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
AHR Antibody
NB100-128
Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.
(1 Review)
(12 Publications)
Species: Hu, Mu, Rt
Applications: GS, ICC/IF, IHC, IHC-P
Cytochrome P450
1A1/1A2 Antibody (MC1
NB100-74398
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunohistochemistry-Paraffin: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis showing positive staining in the cytoplasm and membrane of Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Pm
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AKR1A1 Antibody
(OTI6E3)
NBP2-02164
Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.
Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
FALDH Antibody
(OTI2A7)
NBP2-01017
Western Blot: FALDH Antibody (2A7) [NBP2-01017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FALDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FALDH.
Immunohistochemistry-Paraffin: FALDH Antibody (2A7) [NBP2-01017] Staining of paraffin-embedded Human endometrium tissue using anti-FALDH mouse monoclonal antibody.
Flow Cytometry: FALDH Antibody (2A7) [NBP2-01017] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FALDH antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
alcohol
dehydrogenase 1C Anti
NBP2-92824
Western Blot: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Analysis of extracts of various cell lines, using alcohol dehydrogenase 1C.Exposure time: 10s.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human stomach using alcohol dehydrogenase 1C.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human esophagus using alcohol dehydrogenase 1C.
Species: Hu, Mu, Rt
Applications: WB, IHC
ALDH3B1 Antibody
H00000221-D01P
Western Blot: ALDH3B1 Antibody [H00000221-D01P] - Analysis of ALDH3B1 expression in mouse brain.
Western Blot: ALDH3B1 Antibody [H00000221-D01P] - Analysis of ALDH3B1 expression in MCF-7.
Western Blot: ALDH3B1 Antibody [H00000221-D01P] - Analysis of ALDH3B1 expression in human kidney.
Species: Hu, Mu
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ALDH9A1 Antibody
NBP1-89150
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Analysis in rat cell line NBT-II.
Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Analysis in human cell line TD47D.
(3 Publications)
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CYP1B1 Antibody
NBP2-24722
Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of human adrenal using this antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.
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AF009
Neurophysin II/Arg-vasopressin Antibody [Unconjugated]
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Applications: WB, IHC
Cytochrome P450
1A2 Antibody (OTI8F1)
NBP2-02563
Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 1A2.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2.
Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
alcohol
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Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY alcohol dehydrogenase 1B.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lymph node tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
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ALDH1A2 Antibody
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Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues. Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in cells in endometrial stroma.
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-90120AF350
Alexa Fluor 405 NBP2-90120AF405
Alexa Fluor 488 NBP2-90120AF488
Alexa Fluor 532 NBP2-90120AF532
Alexa Fluor 594 NBP2-90120AF594
Alexa Fluor 647 NBP2-90120AF647
Alexa Fluor 700 NBP2-90120AF700
Alexa Fluor 750 NBP2-90120AF750
Biotin NBP2-90120B
DyLight 350 NBP2-90120UV
DyLight 405 NBP2-90120V
DyLight 488 NBP2-90120G
DyLight 550 NBP2-90120R
DyLight 594 NBP2-90120DL594
DyLight 650 NBP2-90120C
DyLight 680 NBP2-90120FR
DyLight 755 NBP2-90120IR
FITC NBP2-90120F
HRP NBP2-90120H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ALDH3A1