Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) - Azide and BSA Free

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of monoclonal antibody to ALDH1A1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml

Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH1A1 rabbit polyclonal antibody.

ELISA: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALDH1A1 (AAH01505, 1 a.a. ~ 501 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTVKISQKNS
• Marker: Astrocyte Marker
• Specificity: ALDH1A1 - aldehyde dehydrogenase 1 family, member A1 (1G6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) - Azide and BSA Free

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (H00000216-M05)(1)

Publication using H00000216-M05

• Stephanie S, Denise H, Analiz R et al. The Presence and Potential Role of ALDH1A2 in the Glioblastoma Microenvironment. Cells. 2021-09-20 [PMID: 34572134]

Review for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (H00000216-M05) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/02/2023

Summary

• Application: Western Blot
• Sample Tested: Human pancreas/duct epithelioid carcinoma cell line - PANC1 Cells (ATCC # CRL-1469)
• Species: Human
• Lot: G6141-1G6

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Bioinformatics

• Gene Symbol: ALDH1A1
• Entrez:
  • 216(Human)
• Uniprot:
  • AAH01505(Human)