alcohol dehydrogenase Antibody

Images

 
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase ...read more
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase ...read more
Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates ...read more

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

alcohol dehydrogenase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Full length native protein (purified): Alcohol Dehydrogenase (Yeast)
Localization
Cytoplasmic
Specificity
Recognises Alcohol Dehydrogenase. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADH1A
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100

Reactivity Notes

Cross-reacts with S. cerevisiae. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for alcohol dehydrogenase Antibody

  • ADH, alpha subunit
  • ADH1alcohol dehydrogenase 1A
  • alcohol dehydrogenase 1 (class I), alpha polypeptide
  • alcohol dehydrogenase 1A (class I), alpha polypeptide
  • Alcohol dehydrogenase subunit alpha
  • aldehyde reductase
  • EC 1.1.1
  • EC 1.1.1.1

Background

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADH1A