alcohol dehydrogenase 7 Antibody

Product Discontinued

alcohol dehydrogenase 7 Antibody Summary

• Immunogen: Peptide with sequence CISPKDSTKPISE corresponding to internal region according to NP_000664.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADH7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 26 kDa band in lysates of human duodenum and liver and of rat stomach after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5 kDa band according to NP_000664.2. The 26 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for alcohol dehydrogenase 7 Antibody

• ADH4
• ADH-4
• alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• alcohol dehydrogenase class 4 mu/sigma chain
• Alcohol dehydrogenase class IV mu/sigma chain
• alcohol dehydrogenase VII
• alcohol dehydrogenase-7
• class IV sigma-1 alcohol dehydrogenase
• class IV sigmasigma alcohol dehydrogenase
• EC 1.1.1
• EC 1.1.1.1
• Gastric alcohol dehydrogenase
• Retinol dehydrogenase

Background

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alcohol dehydrogenase 7 Antibody (NB100-93368)(1)

Publication using NB100-93368

• Zgombic-Knight M, Foglio MH, Duester G. Genomic structure and expression of the ADH7 gene encoding human class IV alcohol dehydrogenase, the form most efficient for retinol metabolism in vitro. J Biol Chem 1995-03-03 [PMID: 7876191]

Bioinformatics

• Gene Symbol: ADH7
• Entrez:
  • 131(Human)
  • 11529(Mouse)
• Uniprot:
  • P40394(Human)