Human Albumin ELISA Kit (Colorimetric)

Human Albumin ELISA Kit (Colorimetric) [NBP2-60093] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and urine
• Standard Curve Range: 0.078 ug/mL - 5 ug/mL
• Sensitivity: 0.019 ug/mL

Human Albumin ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human albumin. No significant cross-reactivity or interference between human albumin and analogues was observed.
• Standard Curve Range: 0.078 ug/mL - 5 ug/mL
• Sensitivity: 0.019 ug/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ALB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Albumin ELISA Kit (Colorimetric)

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for human Albumin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated Albumin. A competitive inhibition reaction is launched between Albumin (Standards or samples) and biotin-conjugated Albumin with the pre-coated Albumin antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of Albumin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ALB