Albumin Antibody

Immunohistochemistry-Paraffin: Albumin Antibody [NB600-570] - Formalin fixed paraffin embedded human liver stained with Albumin antibody (NB600-570)

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Albumin Antibody Summary

• Immunogen: Purified human albumin.
• Localization: Cytoplasmic
• Specificity: This reacts with human albumin. This is essentially free of other rabbit serum proteins. Specificity is determined by Ouchterlony Double Diffusion (ODD) and immunoelectrophoresis (IEP) versus human serum and human albumin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended incubation time of 30 min at RT.
• Control:

  Human Serum Albumin Overexpression Lysate

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Albumin Antibody

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB
• Entrez:
  • 213(Human)
• Uniprot:
  • P02768(Human)