Albumin Antibody

Product Discontinued

Albumin Antibody Summary

• Immunogen: Human serum albumin purified from Cohn Fraction V.
• Localization: Secreted
• Specificity: This product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human albumin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ALB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Radioimmunodiffusion
• Application Notes: NB120-8940 is useful in Radioimmunodiffusion 2uL antiserum/cm^2 gel or 5uL 1/200 - 1/2000 dilution human serum. IHC (PMID: 22384083)
• Control:

  Human Serum Albumin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Glycine Buffered Saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA, 1 mM EDTA
• Preservative: 0.09% Sodium Azide
• Concentration: 19.0 mg/ml
• Purity: IgG purified

Alternate Names for Albumin Antibody

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (NB120-8940)(3)

Publications using NB120-8940

• Endo Y, Zhang M, Yamaji S, Cang Y. Genetic abolishment of hepatocyte proliferation activates hepatic stem cells. PLoS One;7(2):e31846. 2012-01-01 [PMID: 22384083]
• Bandapalli OR, Hariri N, Macher-Goppinger S et al. Increased apoptosis of host cells and tumor cells in the invasion front of colorectal liver metastases. Anticancer Res. 2011-04-01 [PMID: 21508368] (IF/IHC, Human)
• Brantley JG et al. Cux-1 transgenic mice develop glomerulosclerosis interstitial fibrosis. Kidney Int 63:1240-8. 2003-01-01 [PMID: 12631340]

Bioinformatics

• Gene Symbol: ALB