Alanyl tRNA synthetase Antibody - BSA Free

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706] - Paraffin-embedded hepatoma; antibody dilution 1:500.

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000

Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (50 ug of whole cell lysate) A: Mouse liver 5% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Alanyl tRNA synthetase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Alanyl tRNA synthetase. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AARS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Alanyl tRNA synthetase Antibody - BSA Free

• alanine tRNA ligase 1, cytoplasmic
• Alanine--tRNA ligase
• alanyl-tRNA synthetase
• alanyl-tRNA synthetase, cytoplasmic
• alaRS
• CMT2N
• EC 6.1.1
• EC 6.1.1.7
• Renal carcinoma antigen NY-REN-42

Background

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AARS1
• Entrez:
  • 16(Human)
• Uniprot:
  • P49588(Human)