Alanyl tRNA synthetase Antibodies
We offer Alanyl tRNA synthetase Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Alanyl tRNA synthetase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Alanyl tRNA synthetase Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the Alanyl tRNA synthetase Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Alanyl tRNA synthetase polyclonal antibodies.
Alternate Names for Alanyl tRNA synthetase Antibodies
anti-Alanyl tRNA synthetase antibody, anti-AARS antibody, anti-alanine tRNA ligase 1, cytoplasmic antibody, anti-Alanine--tRNA ligase antibody, anti-alanyl-tRNA synthetase antibody, anti-alanyl-tRNA synthetase, cytoplasmic antibody, anti-AlaRS antibody, anti-CMT2N antibody, anti-EC 6.1.1 antibody, anti-EC 6.1.1.7 antibody, anti-Renal carcinoma antigen NY-REN-42 antibody
12 Results for "alanyl-trna-synthetase" in Primary Antibodies
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414112831.jpg "Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414123897.jpg)
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202415121395.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP3-35492] - Western blot analysis of various lysates using Alanyl tRNA synthetase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202415111883.jpg)
![Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunofluorescence analysis of 293T cells using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414213733.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A: NIH3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP3-12770-img0005.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A: NIH3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12770-img0001.jpg)
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of mouse AARS in mouse kidney with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-12770-img0003.jpg)
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of mouse AARS in mouse stomach with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-12770-img0002.jpg)
![Immunoprecipitation: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-Mouse AARS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-Mouse AARS rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 107 kDaObserved band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP3-12770-img0004.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis using Anti-AARS antibody NBP1-87414 (A) shows similar pattern to independent antibody NBP2-58166 (B).](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis using Anti-AARS antibody NBP1-87414 (A) shows similar pattern to independent antibody NBP2-58166 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0009.jpg)
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP1-87414] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-87414-img0001.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0007.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-57136] - Sample Type: Jurkat Antibody Dilution: 1.0 ug/ml AARS is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-57136-img0008.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP1-57136] - Sample Type: Jurkat Antibody Dilution: 1.0 ug/ml AARS is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-57136] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell processes of pinealocytes Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP1-57136-img0007.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-57136] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate AARS is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-57136-img0009.jpg)
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-57136] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP1-57136-img0005.jpg)
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-57136] - Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec Protocol located in Reviews and Data.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP1-57136-img0006.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-31706-img0005.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706] - Paraffin-embedded hepatoma; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP1-31706-img0003.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-31706-img0002.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (50 ug of whole cell lysate) A: Mouse liver 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-31706-img0004.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166] - Analysis using Anti-AARS antibody NBP2-58166 (A) shows similar pattern to independent antibody NBP1-87414 (B).](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-58166-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166] - Analysis using Anti-AARS antibody NBP2-58166 (A) shows similar pattern to independent antibody NBP1-87414 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP2-58166] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58166-img0001.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-15332] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-15332-img0002.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP2-15332] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP2-15332] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-15332-img0001.jpg)
![Western Blot:[NBP3-30081]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30081_rabbit-alanyl-trna-synthetase-pab-23120251252862.jpg "Western Blot:[NBP3-30081]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.")
![Western Blot:[NBP3-30487]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30487_rabbit-alanyl-trna-synthetase-pab-231202511585565.jpg "Western Blot:[NBP3-30487]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.")
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-97723] - Anti-Alanyl tRNA synthetase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-97723-img0003.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP2-97723] - Anti-Alanyl tRNA synthetase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa")
![Immunoprecipitation: Alanyl tRNA synthetase Antibody [NBP2-97723] - Alanyl tRNA synthetase was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate. 4 ul anti-Alanyl tRNA synthetase rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Alanyl tRNA synthetase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-97723-img0004.jpg)