AKT3 Antibody (199864)

Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB
• Clone: 199864
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AKT3 Antibody (199864) Summary

• Immunogen: E. coli-derived recombinant human Akt3
  Met1-Glu479
  Accession # Q9Y243
• Specificity: Detects endogenous human Akt3 in Western blots. Using Western blots, this antibody does not detect recombinant human Akt1 or Akt2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKT3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AKT3 Antibody (199864)

• Akt3
• Akt-3
• EC 2.7.11
• EC 2.7.11.1
• PKB gamma
• PKBGDKFZp434N0250
• PRKBG
• RAC-gamma
• v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

Background

Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma ) and RAC-gamma serine/threonine kinase (RAC-PK-gamma ), is a member of the Akt family that also includes Akt1 (PKB alpha ) and Akt2 (PKB beta ). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKT3 Antibody (MAB1463)(2)

Publications using MAB1463

• Kathrin Zitzmann, George Vlotides, Stephan Brand, Harald Lahm, Gerald Spöttl, Burkhard Göke et al. Perifosine-mediated Akt inhibition in neuroendocrine tumor cells: role of specific Akt isoforms Endocrine-Related Cancer 2012-06-01 [PMID: 22499437]
• Kathrin Zitzmann, George Vlotides, Stephan Brand, Harald Lahm, Gerald Spöttl, Burkhard Göke et al. Perifosine-mediated Akt inhibition in neuroendocrine tumor cells: role of specific Akt isoforms Endocrine-Related Cancer 2012-06-01 [PMID: 22499437] (Immunohistochemistry, Western Blot, Human)

Bioinformatics

• Gene Symbol: AKT3
• Uniprot:
  • Q9Y243()