AKR1D1 Antibody (1C2) - Azide and BSA Free

Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03] - Analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2. Lane 1: AKR1D1 transfected lysatE (37.4 KDa). Lane 2: Non-transfected lysate.

Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03] - Western blot analysis of AKR1D1 over-expressed 293 cell line. Lane 2 cotransfected with AKR1D1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00006718-M03. GAPDH (36.1 kDa) used as loading control.

ELISA: AKR1D1 Antibody (1C2) [H00006718-M03] - Detection limit for recombinant GST tagged AKR1D1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 1C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AKR1D1 Antibody (1C2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AKR1D1 (NP_005980, 227 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY
• Specificity: AKR1D1 (1C2)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKR1D1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKR1D1 Antibody (1C2) - Azide and BSA Free

• 3o5bred
• Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase
• aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase)
• CBAS2
• Delta(4)-3-oxosteroid 5-beta-reductase
• EC 1.3.1.3
• SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)

Background

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1D1
• Entrez:
  • 6718(Human)
• OMIM:
  • 604741(Human)
• Uniprot:
  • NP_005980(Human)