AKAP9 Antibody (17G10)

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: AKAP9 Antibody (17G10) [NB100-74351] - Normal biopsies of deparaffinized Human pancreas tissue at a dilution of 1:20.

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 17G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AKAP9 Antibody (17G10) Summary

• Immunogen: Synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450.
• Specificity: AKAP450 (17G10)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKAP9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 4 ug/mL
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for AKAP9 Antibody (17G10)

• A kinase (PRKA) anchor protein (yotiao) 9
• AKAP 350
• AKAP 450
• AKAP350A-kinase anchor protein 450 kDa
• AKAP450A-kinase anchor protein 350 kDa
• AKAP-9
• Centrosome- and Golgi-localized PKN-associated protein
• CG-NAPAKAP 120-like protein
• hgAKAP 350
• HYPERION
• KIAA0803A-kinase anchor protein 9
• MU-RMS-40.16A
• PRKA9AKAP9-BRAF fusion protein
• Protein hyperion
• protein kinase A anchoring protein 9
• Protein kinase A-anchoring protein 9
• Protein yotiao
• YOTIAOkinase N-associated protein

Background

AKAP450 (A-Kinase anchoring protein 450) is a coiled-coil, scaffolding protein. It is a member of a group of AKAP's, derived from a spliced gene located on human choromosome 7. AKAP450 regulates kinases which participate in centrosomal structure and regulation. AKAP450 also functions as an MR-1 binding protein with potential action in cytoskeletal attachment of the NMDA receptor. It has also been found to be specifically concentrated at the skeletal muscle and neuromuscular junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP9 Antibody (NB100-74351)(1)

Publication using NB100-74351

• Gorshkov K, Mehta S, Ramamurthy S et al. AKAP-mediated feedback control of cAMP gradients in developing hippocampal neurons. Nat. Chem. Biol. 2017-02-13 [PMID: 28192412] (ICC/IF)

Bioinformatics

• Gene Symbol: AKAP9
• Entrez:
  • 10142(Human)