AHSA1 Antibody (25F2.D10) - Azide and BSA Free

Western Blot: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12884) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).

Immunohistochemistry: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12884) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.

Immunoprecipitation: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Immunoprecipitation analysis using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D10. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 25F2.D10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.59 mg/ml

AHSA1 Antibody (25F2.D10) - Azide and BSA Free Summary

• Immunogen: Mouse Aha1
• Localization: Cytoplasm , Endoplasmic Reticulum
• Specificity: Detects approx 38kDa. Can run up to 45kDa on SDS Page.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: AHSA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:1000
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of AHA1 Antibody was sufficient for detection of Aha1 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Potassium Phosphate, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1.59 mg/ml
• Purity: Protein G purified

Alternate Names for AHSA1 Antibody (25F2.D10) - Azide and BSA Free

• activator of 90 kDa heat shock protein ATPase homolog 1
• AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• C14orf3
• chromosome 14 open reading frame 3
• p38AHA1

Background

1 ug/mL was sufficient for detection of Aha1 in 10ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHSA1