AGXT Antibody

Product Discontinued

AGXT Antibody Summary

• Immunogen: Peptide with sequence C-DKAKSKVYSRKTK corresponding to internal region according to NP_085914.1.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGXT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 22 kDa band in mouse and rat liver lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.4 kDa band according to rat NP_085914.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for AGXT Antibody

• AGT1L-alanine: glyoxylate aminotransferase 1
• AGThepatic peroxisomal alanine:glyoxylate aminotransferase
• AGXT1
• alanine-glyoxylate aminotransferase
• Alanine--glyoxylate aminotransferase
• EC 2.6.1.44
• EC 2.6.1.51
• PH1
• serine:pyruvate aminotransferase
• SPATserine-pyruvate aminotransferase
• SPTserine--pyruvate aminotransferase
• TLH6

Background

AGXT, also known as Serine--pyruvate aminotransferase, is 43kDa and 392 amino acids long. AGXT is localized to the peroxisome and is only expressed in the liver. AGXT is implicated in glyoxylate detoxification and mutations in the AGXT gene have been linked with type I primary hyperoxaluria. AGXT has been shown to have interactions with AGXT2, PEX5, ALAS2, 14-3-3 epsilon and Synphilin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGXT Antibody (NBP1-36933)(1)

Publication using NBP1-36933

• Hoshino J, Kuhne U, Filjak B, Kroger H. Regulation and characterization of L-serine: pyruvate aminotransferase in rat liver cytosol and mitochondria. Z Naturforsch C 1977-03-01 [PMID: 17958]

Bioinformatics

• Gene Symbol: AGXT
• Entrez:
  • 24792(Rat)
• Uniprot:
  • NP_085914(Rat)