Recombinant Human AGTR-2 GST (N-Term) Protein

SDS-Page: Recombinant Human AGTR-2 Protein [H00000186-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human AGTR-2 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-363 of Human AGTR2
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MKGNSTLATTSKNITSGLHFGLVNISGNNESTLNCSQKPSDKHLDAIPILYYIIFVIGFLVNIVVVTLFCCQKGPKKVSSIYIFNLAVADLLLLATLPLWATYYSYRYDWLFGPVMCKVFGSFLTLNMFASIFFITCMSVDRYQSVIYPFLSQRRNPWQASYIVPLVWCMACLSSLPTFYFRDVRTIEYLGVNACIMAFPPEKYAQWSAGIALMKNILGFIIPLIFIATCYFGIRKHLLKTNSYGKNRITRDQVLKMAAAVVLAFIICWLPFHVLTFLDALAWMGVINSCEVIAVIDLALPFAILLGFTNSCVNPFLYCFVGNRFQQKLRSVFRVPITWLQGKRESMSCRKSSSLREMETFVS

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: AGTR2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 67.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human AGTR-2 GST (N-Term) Protein

• AGTR2
• AGTR-2
• Angiostensin Receptor
• angiotensin II receptor, type 2
• Angiotensin II type-2 receptor
• angiotensin receptor 2
• AT2
• AT2ATGR2
• AT2R
• MRX88
• MRX88type-2 angiotensin II receptor

Background

Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G-protein coupled receptors. It is an intergral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for AGTR-2 Recombinant Protein (H00000186-P01)(3)

Publications using H00000186-P01

• Cosarderelioglu C, Kreimer S, Plaza-Rodriguez AI et al. Decoding Angiotensin Receptors: TOMAHAQ-Based Detection and Quantification of Angiotensin Type-1 and Type-2 Receptors Journal of the American Heart Association 2023-09-19 [PMID: 37681524]
• Shivapathasundram G, Wickremesekera A, Brasch H et al. Expression of Components of the Renin-Angiotensin System by the Putative Stem Cell Population Within WHO Grade I Meningioma Front. Surg. 2019-05-16 [PMID: 31157231] (WB, Human)
• Papali'i-Curtin, JC;Brasch, HD;van Schaijik, B;de Jongh, J;Marsh, RW;Tan, ST;Itinteang, T; Expression of Components of the Renin-Angiotensin System in Pyogenic Granuloma Front Surg 2019-04-09 [PMID: 31024924] (WB, Human)

Review for AGTR-2 Recombinant Protein (H00000186-P01) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Verified Customer SDS-PAGE Control 06/30/2017

Summary

• Application: SDS-PAGE Control
• Comments: A slight high molecular weight smear with HRP western blots, otherwise a well staining single band at an expected size

Bioinformatics

• Gene Symbol: AGTR2