AGTR-2 Overexpression Lysate (Native)

Images

 
Western Blot: Angiotensin II Type 2 Receptor Overexpression Lysate (Adult Normal) [NBL1-07397] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Angiotensin II ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

AGTR-2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223317 and based on accession number NM_000686. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens angiotensin II receptor, type 2 (AGTR2), mRNA.
Gene
AGTR2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for AGTR-2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AGTR-2 Overexpression Lysate (Native)

  • AGTR2
  • AGTR-2
  • angiotensin II receptor, type 2
  • Angiotensin II type-2 receptor
  • angiotensin receptor 2
  • AT2
  • AT2ATGR2
  • AT2R
  • MRX88
  • MRX88type-2 angiotensin II receptor

Background

Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G-protein coupled receptors. It is an intergral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77078
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-59882
Species: Hu
Applications: WB, IHC
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut
NBP1-06529
Species: Hu
Applications: WB, IHC-P, IP, PLA
NBP1-80789
Species: Hu
Applications: WB, IHC, IHC-P
AF1396
Species: Hu
Applications: WB
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-89200
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P

Publications for AGTR-2 Lysate (NBL1-07397) (0)

There are no publications for AGTR-2 Lysate (NBL1-07397).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AGTR-2 Lysate (NBL1-07397) (0)

There are no reviews for AGTR-2 Lysate (NBL1-07397). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AGTR-2 Lysate (NBL1-07397) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional AGTR-2 Products

Bioinformatics Tool for AGTR-2 Lysate (NBL1-07397)

Discover related pathways, diseases and genes to AGTR-2 Lysate (NBL1-07397). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AGTR-2 Lysate (NBL1-07397)

Discover more about diseases related to AGTR-2 Lysate (NBL1-07397).
 

Pathways for AGTR-2 Lysate (NBL1-07397)

View related products by pathway.

PTMs for AGTR-2 Lysate (NBL1-07397)

Learn more about PTMs related to AGTR-2 Lysate (NBL1-07397).
 

Research Areas for AGTR-2 Lysate (NBL1-07397)

Find related products by research area.

Blogs on AGTR-2

There are no specific blogs for AGTR-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

AGTR-2 Antibody
NBP1-77368

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AGTR-2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol AGTR2
Entrez