Aggrecan Antibody (CS-56)

Immunocytochemistry/Immunofluorescence: Aggrecan Antibody (CS-56) [NB120-11570] - Coronal section of the developing cerebral cortex of an embryonic day 16 rat stained with Monoclonal Anti-Chondroitin Sulfate followed by fluorescein labeled Goat Anti-Mouse Ig. The chondroitin sulfate proteoglycan (CSPG) is most concentrated in the pia matter and the sub-plate (bright green layers). There is moderate staining in the intermediate zone, and there is very little CSPG in the cortical plate and ventricular zone. Data supplied by B. Miller, Washington Univ. School of Medicine, St. Louis, MO.

Immunohistochemistry: Aggrecan Antibody (CS-56) [NB120-11570] - Long-term load duration decreased expression of NP matrix macromolecules in porcine disc NP cells. Immunohistochemistry staining of aggrecan and collagen II. Magnification: 200x, scale =100 um. The results showed that long-term load duration (8 h per day) significantly decreased gene expression of matrix molecules (aggrecan and collagen II) and their protein deposition within the NP tissue compared with the short-term load duration (1 h per day) and the control (non-compression). Data are expressed as the means +/- S.D., n=3. #: indicates a significant difference (P<0.05) when compared with the control group. *: indicates a significant difference between two groups (P<0.05). Image collected and cropped by CiteAb from the following publication (//portlandpress.com/bioscirep/article-lookup/doi/10.1042/BSR20160582) licensed under a CC-BY license.

Immunohistochemistry-Frozen: Aggrecan Antibody (CS-56) [NB120-11570] - staining of a frozen section of normal rabbit aorta. Data supplied by Z. Gallis, McGill University, Montreal.

Electron Microscopy: Aggrecan Antibody (CS-56) [NB120-11570] - Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate (NB120-11570) and Goat Anti-Mouse IgM (u-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen). Data supplied by Z. Gallis, McGill University, Montreal.

Product Discontinued

Aggrecan Antibody (CS-56) Summary

• Immunogen: Ventral membranes of chicken gizzard fibroblasts.
• Marker: Chondrocyte Marker
• Specificity: The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Microarray
  • Western Blot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33032177).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Aggrecan Antibody (CS-56)

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aggrecan Antibody (NB120-11570)(11)

Publications using NB120-11570

• Xu J, Fang Q, Liu Y et al. In situ ornamenting poly(epsilon-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells Colloids Surf B Biointerfaces 2020-10-01 [PMID: 33032177] (ICC/IF, Rabbit)
• Zhan JW, Wang SQ, Feng MS et al. Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model PLoS ONE 2020-06-25 [PMID: 32584845] (IF/IHC, Rabbit)
• Jiang Y, Fu L, Song Y. Responses of apoptosis and matrix metabolism of annulus fibrosus cells to different magnitudes of mechanical tension in vitro Biosci. Rep. 2019-02-28 [PMID: 30700570] (WB, Rat)
• Shi J, Pang L, Jiao S. The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture. Biosci. Rep. 2018-04-27 [PMID: 29437905] (IHC-P, Porcine)
• Zhou H, Shi J, Zhang C, Li P. Static compression down-regulates N-cadherin expression and facilitates loss of cell phenotype of nucleus pulposus cells in a disc perfusion culture. Biosci. Rep. 2018-02-28 [PMID: 29273678] (IF/IHC, Porcine)
• Gan Y, Li P, Wang L et al. An interpenetrating network-strengthened and toughened hydrogel that supports cell-based nucleus pulposus regeneration. Biomaterials 2017-08-01 [PMID: 28505597]
• Li P, Zhang R, Wang L et al. Long-term load duration induces N-cadherin down-regulation and loss of cell phenotype of nucleus pulposus cells in a disc bioreactor culture. Biosci. Rep. 2017-04-30 [PMID: 28351894] (WB, Porcine)
  
  Details:
  This aggrecan antibody was used to examine the effect long term load on the expression of NP matrix molecules in pig disc NP cells.
• Li P, Gan Y et al. 17beta-estradiol Attenuates TNF-alpha-Induced Premature Senescence of Nucleus Pulposus Cells through Regulating the ROS/NF-kapa B Pathway. Int J Biol Sci 2017-04-03 [PMID: 28255267] (ICC/IF, Rat)
• Li P, Gan Y, Xu Y et al. Matrix homeostasis within the immature annulus fibrosus depends on the frequency of dynamic compression: a study based on the self-developed mechanically active bioreactor. Biomech Model Mechanobiol 2016-09-02 [PMID: 27590020] (IF/IHC)
• Li P, Gan Y, Wang H et al. Biological Responses of the Immature Annulus Fibrosus to Dynamic Compression in a Disc Perfusion Culture Cells Tissues Organs. 2016-07-15 [PMID: 27415834]
• Li P, Shi R, Chen D et al. Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture. BMC Musculoskelet Disord 2016-03-22 [PMID: 27000557]

FAQs for Aggrecan Antibody (NB120-11570). (Showing 1 - 1 of 1 FAQs).

1. Can I use NB120-11570 for IHC-paraffin?
   • Our product NB120-11570 has not yet been tested in paraffin embedded tissues, only in frozen tissues. So we unfortunately have not been able to add IHC-P to our verified list of applications for this product.

Bioinformatics

• Gene Symbol: ACAN
• Entrez:
  • 176(Human)
  • 11595(Mouse)
  • 58968(Rat)
• Uniprot:
  • P16112()