Aggrecan Antibody (BC-13)

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Product Details

Summary
Reactivity Hu, Po, Bv, Ca, EqSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
BC-13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Aggrecan Antibody (BC-13) Summary

Immunogen
...NITEGE synthetic peptide conjugate.
Marker
Chondrocyte Marker
Specificity
Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope...TEGE after cleavage between amino acids..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACAN
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB110-12050 in the following applications:

Reactivity Notes

Human, porcine, bovine, equine and canine tissues. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Purity
Tissue culture supernatant

Notes

Please note: this product has dropped from 2.0 ml to 1.0 ml, however, it has also been purified and can be used at higher concentration. Additionally, the buffer is now only PBS and Sodium Azide, and the BSA has been removed. Please contact our customer support with any questions.

Alternate Names for Aggrecan Antibody (BC-13)

  • ACAN
  • AGC1
  • AGC1SEDK
  • aggrecan core protein
  • Aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16
  • MSK16AGCAN
  • SEDK

Background

This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aggrecan Antibody (NB110-12050)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC-P.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aggrecan Antibody (NB110-12050) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Aggrecan Antibody (NB110-12050)

Discover related pathways, diseases and genes to Aggrecan Antibody (NB110-12050). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Aggrecan Antibody (NB110-12050)

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Blogs on Aggrecan.

Aches & Pains: Aggrecan in Joint Disease and Osteoarthritis
Aggrecan is essential for the normal function of articular cartilage and intervertebral discs. Aggrecan provides the ability for the tissues to withstand compressive loading. This property is dependent on both the high charge density endowed by its nu...  Read full blog post.

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Bioinformatics

Gene Symbol ACAN
Entrez