Aggrecan Antibody (BC-13)

Product Discontinued

Aggrecan Antibody (BC-13) Summary

• Immunogen: ...NITEGE synthetic peptide conjugate.
• Marker: Chondrocyte Marker
• Specificity: Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated C-terminal neoepitope...TEGE after cleavage between amino acids..EGE373 and 374ARG.. within the interglobular domain of aggrecanase-catabolised aggrecan.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAN
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:50
• Application Notes: Optimal dilutions/concentrations should be determined by the end user. This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, porcine, bovine, equine and canine tissues. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.02% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Please note: this product has dropped from 2.0 ml to 1.0 ml, however, it has also been purified and can be used at higher concentration. Additionally, the buffer is now only PBS and Sodium Azide, and the BSA has been removed. Please contact our customer support with any questions.

Alternate Names for Aggrecan Antibody (BC-13)

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aggrecan Antibody (NB110-12050)(1)

Publication using NB110-12050

• Bouhenni R, Hart M, Al-Jastaneiah S et al. Immunohistochemical expression and distribution of proteoglycans and collagens in sclerocornea. Int Ophthalmol. 2013-01-01 [PMID: 23325424] (IHC-P, ICC/IF, Human)

Bioinformatics

• Gene Symbol: ACAN
• Entrez:
  • 176(Human)