AGAP2 Antibody - BSA Free

Western Blot: AGAP2 Antibody [NBP1-77194] - Analysis of AGAP2 in mouse brain cell lysates with anti-AGAP2 (CT) using this antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry: AGAP2 Antibody - BSA Free [NBP1-77194] - Immunohistochemistry of AGAP2 in mouse brain tissue with AGAP2 antibody at 10 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AGAP2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE. The immunogen is located within the last 50 amino acids of PIKE. Amino Acid Squence: PRRRSSAASVGRADAP
• Specificity: Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGAP2
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  AGAP2 Peptide (NBP1-77194PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for AGAP2 Antibody - BSA Free

• AGAP-2
• ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
• centaurin, gamma 1
• centaurin-gamma-1
• CENTG1
• cnt-g1
• FLJ16430
• GGAP2
• GTP-binding and GTPase activating protein 2
• GTP-binding and GTPase-activating protein 2
• KIAA0167
• Phosphatidylinositol-3-kinase enhancer
• phosphoinositide 3-kinase enhancer
• PIKEArf GAP with GTP-binding protein-like, ANK repeat and PH domains 2

Background

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGAP2
• Uniprot:
  • AAM97540(Human)