AG-2/AGR2 Antibody (SN74-01)

Western Blot: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Western blot analysis of AG-2/AGR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti

Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.

Flow Cytometry: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: AG-2/AGR2 Antibody (SN74-01) [NBP2-67673] - Staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

AG-2/AGR2 Antibody (SN74-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human AG-2/AGR2 aa 1-50 / 175. (SwissProt: O95994 Human; SwissProt: O88312 Mouse)
• Localization: Secreted, Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AG-2/AGR2 Antibody (SN74-01)

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

Anterior gradient homolog 2 (AGR2) is a proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. AGR2 is expressed in a number of different tumor types and high levels of AGR2 correlate with downregulation of the p53 response. AGR2 is being investigated for its use as a potential diagnostic and therapeutic target in various cancers, and AGR2 antibodies are important tools for cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGR2