AG-2/AGR2 Antibody (EPR3278)

Product Discontinued

AG-2/AGR2 Antibody (EPR3278) Summary

• Immunogen: A synthetic peptide corresponding to residues in human AGR2 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGR2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:20
  • Western Blot 1:5000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 17kDa.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for AG-2/AGR2 Antibody (EPR3278)

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. AGR2 is expressed in a number of different tumor types and is being investigated for its use as a potential diagnostic and therapeutic target in various cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AG-2/AGR2 Antibody (NBP1-40630)(1)

Publication using NBP1-40630

• Cocce KJ, Jasper JS, Desautels TK et al. The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer Cell Rep 2019-10-22 [PMID: 31644911]

FAQs for AG-2/AGR2 Antibody (NBP1-40630). (Showing 1 - 1 of 1 FAQ).

1. What is the detailed concentration for this product and the epitope for this antibody?
   • NBP1-40630 was generated against a synthetic peptide which maps between amino acids 20 and 50 of human AGR2 (Uniprot ID: O95994), and the concentration of the latest lot (YH041606C) is 0.2910mg/ml.

Bioinformatics

• Gene Symbol: AGR2
• Entrez:
  • 10551(Human)
• Uniprot:
  • O95994(Human)