AE Binding Protein 1/ACLP Antibody (590831) [Unconjugated]

HASMC human human aortic smooth muscle cells was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Molt-4 human human cell line was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 590831
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AE Binding Protein 1/ACLP Antibody (590831) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACLP
  Glu345-Phe1158
  Accession # Q8IUX7
• Specificity: Detects human ACLP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ACLP is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AEBP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AE Binding Protein 1/ACLP Antibody (590831) [Unconjugated]

• ACLP
• ACLPadipocyte enhancer binding protein 1
• AE binding protein 1
• AE-binding protein 1adipocyte enhancer-binding protein 1
• AEBP1
• Aortic carboxypeptidase-like protein
• FLJ33612

Background

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1), is a 175 kDa molecule that contains a discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942). It is expressed in proliferating adipocytes and represses the transcription of FABP4. ACLP promotes macrophage cholesterol retention, the formation of foam cells, and inflammatory responses. It is also an extracellular matrix-associated protein found in fibrotic tissues including vascular smooth muscle. An alternately spliced isoform lacks aa 1-457 and has an altered sequence at its N-terminus. Over aa 345-1158, human ACLP shares 88% aa sequence identity with mouse and rat ACLP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AEBP1
• Uniprot:
  • Q8IUX7()