Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, B/N
• Clone: Ig2838
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free (NBP3-09003) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human recombinant A2-adenosine receptor.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADORA2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  
  This antibody binds to the A2 adenosine receptor, a G-protein-coupled receptor for adenosine that, upon ligand binding, activates adenylyl cyclase. The antibody acts as an allosteric inhibitor.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

• A2aR
• adenosine A2 receptor
• Adenosine A2a R
• adenosine A2a receptor
• Adenosine A2aR
• adenosine receptor A2a
• adenosine receptor subtype A2a
• ADORA 2
• ADORA2
• ADORA2A
• hA2aR
• RDC8

Background

Adenosine A2a Receptor encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADORA2A