ADAR Antibody - BSA Free

Western Blot: ADAR Antibody [NBP2-92918] - analysis of 293F, using ADAR1 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Immunohistochemistry-Paraffin: ADAR Antibody [NBP2-92918] -Analysis of paraffin-embedded human colon using ADAR1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ICC/IF-ADAR Antibody-NBP2-92918- Analysis of HepG2 cells using ADAR1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ADAR Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ADAR Antibody - BSA Free (NBP2-92918) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ADAR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-299 of human ADAR1 (NP_001102.2). RPDGHSQGAPNSDPSLE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ADAR Antibody - BSA Free

• ADAR
• ADAR1
• ADAR1DSH
• adenosine deaminase, RNA-specific
• DRADA
• DRADAP136
• DSRAD
• DSRADadenosine deaminase acting on RNA 1-A
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.37
• G1P1
• G1P1double-stranded RNA-specific adenosine deaminase
• IFI4
• IFI-4
• IFI4dsRNA adenosine deaminase
• interferon-induced protein 4
• Interferon-inducible protein 4
• K88DSRBP
• p136
• p136,136 kDa double-stranded RNA-binding protein

Background

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAR Antibody (NBP2-92918)(1)

Publication using NBP2-92918

• Wang P, Huang Z, Peng Y et al. Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair Nature communications Oct 31 2022 [PMID: 36316334] (WB, Human, Mouse)

Bioinformatics

• Gene Symbol: ADAR