ADAMTS4 Antibody (416610) [Unconjugated]

ADAMTS4 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB4307) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IP, B/N, ICC/IF
• Clone: 416610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ADAMTS4 Antibody (416610) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAMTS4
  Phe213-Cys685
  Accession # O75173
• Specificity: Detects human ADAMTS4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ADAMTS1, rhADAMTS5, rhADAMTS13, or rhADAMTSL1.2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAMTS4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS4 Antibody (416610) [Unconjugated]

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
• ADAM metallopeptidase with thrombospondin type 1 motif, 4
• ADAM-TS 4
• ADAMTS-2
• ADAMTS4
• ADAM-TS4
• ADAMTS-4
• ADMP-1
• ADMP-1EC 3.4.24.82
• Aggrecanase 1
• aggrecanase-1
• EC 3.4.24
• KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Background

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). ADAMTS4 consists of a signal peptide (aa 1 to 51), a pro domain (aa 52 to 212) and a mature chain (aa 213 to 837) containing the following domains: catalytic (aa 218 to 425), disintegrin (aa 437 to 519), TSP type-1 (aa 520 to 575), Cys-rich (aa 577 to 685) and spacer (aa 686 to 837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.

1. Totorella, M. D. et al. (1999) Science 284:1664.
2. Porter, S. et al. (2005) Biochem. J. 386:15.
3. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
4. Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554.
5. Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101.
6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
7. Pratta, M. A. et al. (2003) Arthritis Reum. 48:119.
8. Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAMTS4 Antibody (MAB4307)(1)

Publication using MAB4307

• Bing Zhu, Guanxing Cui, Qifu Zhang, Xiankui Cheng, Shusen Tang <p>Desumoylation of aggrecan and collagen II facilitates degradation via aggrecanases in IL-1 beta -mediated osteoarthritis</p> Journal of Pain Research 2019-07-12 [PMID: 31372032]

Bioinformatics

• Gene Symbol: ADAMTS4
• Uniprot:
  • O75173()