ADAMTS3 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

ADAMTS3 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS3
  Ser19-Cys712
  Accession # O15072
• Specificity: Detects human ADAMTS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 3% cross-reactivity with recombinant human (rh) ADAMTS4 and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS5, rhADAMTS13, and rhADAMTSL1.2 i
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS3 Antibody [Alexa Fluor® 350]

• A disintegrin and metalloproteinase with thrombospondin motifs 3
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 3
• ADAM metallopeptidase with thrombospondin type 1 motif, 3
• ADAM-TS 3
• ADAMTS3
• ADAM-TS3
• ADAMTS-3
• ADAMTS-4
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• KIAA0366PC II-NP
• PC II-NP
• Procollagen II amino propeptide-processing enzyme
• Procollagen II N-proteinase
• zinc metalloendopeptidase

Background

ADAMTS3 (A disintegrin and metalloprotease with thrombospondin motifs 3; also PC II-NP) is a 140-150 kDa member of the ADAMTS family of Zn metalloproteases. It is expressed by osteoblasts, chrondrocytes, myoepithelium and fibroblasts, and participates in collagen maturation. Together with ADAMTS2 and TS14, ADAMTS3 is known to process the N-terminus of procollagen. In particular, it cleaves the N-terminal globular region of collagen I and II, leading to fibril formation. Mature human proADAMTS3 is a secreted, 1185 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 38-201), a peptidase M12B domain (aa 256-460), a disintegrin region (aa 470-550), four TSP type I sequences (aa 551-1015), and a PLAC domain (aa 1015-1054). There are two potential splice variants. One shows a 38 aa substitution for aa 169-1205, while another shows an alternative start site at Met171. Over aa 19-712, human ADAMTS3 shares 91% aa identity with mouse ADAMTS3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.