Rat ADAMTS13 ELISA Kit (Chemiluminescence)

ELISA: Rat ADAMTS13 ELISA Kit (Chemiluminescence) [NBP2-66441] - Samples were spiked with high concentrations of Rat ADAMTS13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat ADAMTS13 ELISA Kit (Chemiluminescence) [NBP2-66441] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat ADAMTS13 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Rat ADAMTS13/VWFCP in samples. No significant cross-reactivity or interference between Rat ADAMTS13/VWFCP and analogues was observed.
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.07%
• Intra-Assay: CV% < 10.57%
• Spike Recovery: 87-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: ADAMTS13

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat ADAMTS13 ELISA Kit (Chemiluminescence)

• A disintegrin and metalloproteinase with thrombospondin motifs 13
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13
• ADAM metallopeptidase with thrombospondin type 1 motif, 13
• ADAMTS13
• ADAM-TS13
• ADAMTS-13
• DKFZp434C2322
• EC 3.4.24.14
• EC 3.4.24.82
• EC 3.4.24.87
• FLJ42993
• MGC118899
• MGC118900
• TTP
• TTPADAM-TS 13
• vWF-cleaving protease
• vWF-CP
• vWF-CPC9orf8
• VWFCPvon Willebrand factor-cleaving protease

Background

ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13. The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for ADAMTS13 ELISA Kit (NBP2-66441)(1)

Publication using NBP2-66441

• Liu CD, Liu NN, Zhang S et al. Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway Acta pharmacologica Sinica 2020-12-10 [PMID: 33303991] (ELISA)

FAQs for ADAMTS13 ELISA Kit (NBP2-66441). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADAMTS13