ADAMTS13 Antibody

Western Blot: ADAMTS13 Antibody [NB110-82382] - Mouse liver lysate. Antibody dilution 1:500

Product Discontinued

ADAMTS13 Antibody Summary

• Immunogen: Synthetic peptide derived from Nter domain of ADAM-TS 13 protein
• Specificity: Reacts specifically with human 153 kDa ADAM-TS 13 protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAMTS13
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:500-1:2000
• Theoretical MW: 154 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for ADAMTS13 Antibody

• A disintegrin and metalloproteinase with thrombospondin motifs 13
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13
• ADAM metallopeptidase with thrombospondin type 1 motif, 13
• ADAMTS13
• ADAM-TS13
• ADAMTS-13
• DKFZp434C2322
• EC 3.4.24.14
• EC 3.4.24.82
• EC 3.4.24.87
• FLJ42993
• MGC118899
• MGC118900
• TTP
• TTPADAM-TS 13
• vWF-cleaving protease
• vWF-CP
• vWF-CPC9orf8
• VWFCPvon Willebrand factor-cleaving protease

Background

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigenis. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrands disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAMTS13 Antibody (NB110-82382)(6)

Publications using NB110-82382

• Zhou S, Guo J, Liao X et al. rhADAMTS13 reduces oxidative stress by cleaving VWF in ischaemia/reperfusion-induced acute kidney injury Acta physiologica (Oxford, England) 2022-01-06 [PMID: 34989474]
• Zhou S, Guo J, Zhao L et al. ADAMTS13 inhibits oxidative stress and ameliorates progressive chronic kidney disease following ischaemia/reperfusion injury Acta physiologica (Oxford, England) 2020-11-23 [PMID: 33226724]
• Zhou S, Jiang S et al. ADAMTS13 protects mice against renal ischemia-reperfusion injury by reducing inflammation and improving endothelial function. Am J Physiol Renal Physiol 2019-01-01 [PMID: 30461292] (ICC/IF, Mouse)
• Hershko K, Simhadri V, Blaisdell A et al. Cyclosporin A impairs the secretion and activity of ADAMTS13 J Biol Chem 2012-11-09 [PMID: 23144461] (WB, Mouse, Human)
• Ganburged G, Suda N, Saito M et al. Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice. Cell Tissue Res 2010 Sep [PMID: 20714769] (IHC-P, Mouse)
• Sen U, Tyagi N, Patibla PK et al. Fibrinogen-induced Endothelin-1 Production from Endothelial Cells. Am J Physiol Cell Physiol;296(4):C840-7. 2009-04-01 [PMID: 19193866]

Bioinformatics

• Gene Symbol: ADAMTS13
• Entrez:
  • 11093(Human)
• Uniprot:
  • Q76LX8()