Western blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAMTS1 Monoclonal Antibody (Catalog # MAB2197) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Protein A or G purified from hybridoma culture supernatant
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ADAMTS1 Antibody (268104)
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1
ADAM metallopeptidase with thrombospondin type 1 motif, 1
ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine‑rich, spacer and a variable number of TS type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha -2-macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of cofactor such as fibulin-1 (8). The aggrecanase activity can be inhibited using 5 mM 1,10 Phenanthroline. ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF-2 (11, 12). The purified rhADAMTS1 starts at the N-terminus of the catalytic domain and ends in the beginning of the spacer region.
Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
Porter, S. et al. (2005) Biochem. J. 386:15.
Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
Kuno, K. et al. (2000) FEBS Lett. 478:241.
Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
Lee, N., et al. (2005) J. Biol. Chem. 280:34796.
Shindo, T., et al. (2000) J. Clin. Invest. 105:1345.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.