ADAM9 Knockout HeLa Cell Lysate Summary
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 1
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.
Lysate Details for Array
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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.
Alternate Names for ADAM9 Knockout HeLa Cell Lysate
- a disintegrin and metalloproteinase domain 9 (meltrin gamma)
- ADAM 9
- ADAM metallopeptidase domain 9 (meltrin gamma)
- ADAM metallopeptidase domain 9
- Cellular disintegrin-related protein
- cone rod dystrophy 9
- disintegrin and metalloproteinase domain-containing protein 9
- EC 3.4.24
- EC 3.4.24.-
- Meltrin gamma
- Metalloprotease/disintegrin/cysteine-rich protein 9
- Myeloma cell metalloproteinase
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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