ADAM9 Antibody (138537) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 138537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ADAM9 Antibody (138537) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM9 Ectodomain
  Accession # Q13443
• Specificity: Detects human ADAM9 Ectodomain in Western blots. In Western blots, approximately 10% cross-reactivity with the ectodomain of recombinant mouse ADAM9 is observed and no cross-reactivity with the ectodomain of recombinant human ADAM8, 9, 15, or 17 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM9 Antibody (138537) [Unconjugated]

• a disintegrin and metalloproteinase domain 9 (meltrin gamma)
• ADAM 9
• ADAM metallopeptidase domain 9 (meltrin gamma)
• ADAM metallopeptidase domain 9
• ADAM9
• Cellular disintegrin-related protein
• cone rod dystrophy 9
• CORD9
• disintegrin and metalloproteinase domain-containing protein 9
• EC 3.4.24
• EC 3.4.24.-
• MCMP
• MCMPMDC9KIAA0021Mltng
• MDC9
• Meltrin gamma
• Meltrin-gamma
• Metalloprotease/disintegrin/cysteine-rich protein 9
• MLTNG
• Myeloma cell metalloproteinase

Background

ADAM9, also known as MDC9 or meltrin gamma , is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane-anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha _v beta ₅ and alpha ₆ beta ₁ (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

1. Moss, et al. (2001) DDT 6:417.
2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
3. Izumi, et al. (1998) EMBO J. 17:7260.
4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
5. Nath, et al. (2000) J. Cell Sci. 113:2319.
6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM9 Antibody (MAB939)(8)

Publications using MAB939

• M Ueno, T Shiomi, S Mochizuki, M Chijiiwa, M Shimoda, Y Kanai, F Kataoka, A Hirasawa, N Susumu, D Aoki, Y Okada ADAM9 is over-expressed in human ovarian clear cell carcinomas and suppresses cisplatin-induced cell death Cancer Sci., 2018-02-01;109(2):471-482. 2018-02-01 [PMID: 29247567] (Neutralization, Human)
• CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT Sci Rep, 2017-11-08;7(1):15108. 2017-11-08 [PMID: 29118335] (Neutralization, Human)
• Céline Mongaret Kossmann, Maxime Annereau, Audrey Thomas-Schoemann, Carole Nicco-Overney, Christiane Chéreau, Frédéric Batteux et al. ADAM9 expression promotes an aggressive lung adenocarcinoma phenotype Tumour Biol 7/5/2017 [PMID: 28675123]
• Kuo-Liang Chiu, Ting-Ting Kuo, Qian-Yu Kuok, Yu-Sen Lin, Chung-Hung Hua, Chen-Yuan Lin et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis Scientific Reports 11/10/2015 [PMID: 26553452]
• Kuo-Liang Chiu, Ting-Ting Kuo, Qian-Yu Kuok, Yu-Sen Lin, Chung-Hung Hua, Chen-Yuan Lin et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis Scientific Reports 2015-11-10 [PMID: 26553452] (Western Blot, Human)
• Lin C, Chen H, Huang C, Lai L, Lu T, Tseng G, Kuo T, Kuok Q, Hsu J, Sung S, Hung M, Sher Y ADAM9 promotes lung cancer metastases to brain by a plasminogen activator-based pathway. Cancer Res, 2014-07-24;74(18):5229-43. 2014-07-24 [PMID: 25060522] (Western Blot, Human, Mouse)
• Sung SY, Kubo H, Shigemura K, Arnold RS, Logani S, Wang R, Konaka H, Nakagawa M, Mousses S, Amin M, Anderson C, Johnstone P, Petros JA, Marshall FF, Zhau HE, Chung LW Oxidative stress induces ADAM9 protein expression in human prostate cancer cells. Cancer Res., 2006-10-01;66(19):9519-26. 2006-10-01 [PMID: 17018608] (IHC-P, Western Blot, Human)
• Karadag A, Zhou M, Croucher PI ADAM-9 (MDC-9/meltrin-gamma), a member of the a disintegrin and metalloproteinase family, regulates myeloma-cell-induced interleukin-6 production in osteoblasts by direct interaction with the alpha(v)beta5 integrin. Blood, 2005-12-22;107(8):3271-8. 2005-12-22 [PMID: 16373656] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ADAM9
• Uniprot:
  • Q13443()