ADAM22 Antibody (S57-2)

Product Discontinued

ADAM22 Antibody (S57-2) Summary

• Immunogen: Fusion protein amino acids 444-526 (extracellular disintegrin domain) of mouse ADAM22
• Localization: Membrane
• Specificity: Detects the extracellular domain of ADAM22 approx 90kDa. Does not cross-react with ADAM11. Weakly reactive in human samples.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM22
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1ug/ml
• Application Notes: 1 ug/ml of ADAM22 (extracellular) Antibody was sufficient for detection of ADAM22 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ADAM22 Antibody (S57-2)

• ADAM 22
• ADAM metallopeptidase domain 22
• ADAM22
• MDC2
• metalloproteinase-disintegrin ADAM22-3
• metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
• MGC149832

Background

ADAM22 was first described as MDC2 (Metalloproteinase-like disintergin like cysteine rich 2) from analysis of human brain libraries, in search of brain-specific proteins. Two splice variants with different carboxyterminal ends have been described. ADAM22 participates in cell adhesion and spreading by associating with 14-3-3 proteins. It is constitutively produced in normal brain tissue, but decreased or absent in high grade gliomas. Different splice variants of ADAM22 are differentially expressed in areas of the brain. A complex of ADAM22 and LGI1 were shown to regulate synaptic transmission. Complexes of ADAM22, stargazing, and a range of other proteins anchor ADAM22 into the cytoskeleton, and keep ADAM22 in association with the postsynaptic space. ADAM22 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. ADAM22 is a member of the ADAMS that function in tandem with other ADAMs and which may also have specific individual functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM22 Antibody (NBP2-22425)(1)

Publication using NBP2-22425

• Richter Katharina N, Wildhagen Hanna, Helm Martin S et al. Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins. Scientific Reports 2018-10-04 [PMID: 30287847] (Rat)

Bioinformatics

• Gene Symbol: ADAM22
• Uniprot:
  • Q9R1V6(Mouse)