Human ADAM10 ELISA Kit (Chemiluminescence)

ELISA: Human ADAM10 ELISA Kit (Chemiluminescence) [NBP2-66435] - Samples were spiked with high concentrations of Human ADAM10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ADAM10 ELISA Kit (Chemiluminescence) [NBP2-66435] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human ADAM10 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human ADAM10 in samples. No significant cross reactivity or interference between Human ADAM10 and analogues was observed.
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.77%
• Intra-Assay: CV% < 10.42%
• Spike Recovery: 91-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: ADAM10

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ADAM10 ELISA Kit (Chemiluminescence)

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ADAM10