ADAM10 Antibody (163003) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM10 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # IC1427P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 163003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ADAM10 Antibody (163003) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM10
  Thr214-Glu672
  Accession # O14672
• Specificity: Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM10 Antibody (163003) [Phycoerythrin]

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• KUZ
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

1. Rooke, et al. (1996) Science 273:1227.
2. Pan and Rubin (1997) Cell 90:271.
3. Qi, et al. (1999) Science 283:91.
4. Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
5. Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
6. Chantry, et al. (1989) J. Biol. Chem. 264:21603.
7. Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
8. Chantry and Glynn (1990) Biochem. J. 268:245.
9. Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (IC1427P)(2)

Publications using IC1427P

• H Lécuyer, Z Virion, JP Barnier, S Matczak, S Bourdoulou, E Bianchini, F Saller, D Borgel, X Nassif, M Coureuil An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminans PLoS Pathog., 2018-04-09;14(4):e1006981. 2018-04-09 [PMID: 29630665] (Flow Cytometry, Human)
• Hina Mir, Rajesh Singh, Goetz H. Kloecker, James W. Lillard, Shailesh Singh CXCR6 expression in non-small cell lung carcinoma supports metastatic process via modulating metalloproteinases Oncotarget 2015-04-30 [PMID: 25888629] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O14672()