Actin (Muscle Specific) Antibody (MSA/953)

Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Formalin-fixed paraffin-embedded rat stomach stained with muscle specific actin monoclonal antibody (MSA/953)

Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin format of this antibody.

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)

Flow Cytometry: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Azide and BSA Free format of this antibody.

Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Azide and BSA Free format of this antibody.

• Reactivity: Hu, Rt, Rb
• Applications: WB, Flow, Flow-IC, IHC, IHC-P, ICC/IF
• Clone: MSA/953
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Actin (Muscle Specific) Antibody (MSA/953) Summary

• Immunogen: Synthetic peptide common among human muscle actins
• Localization: Cytoplasmic
• Marker: Muscle Cell Marker
• Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.AIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Flow (Intracellular)
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Actin (Muscle Specific) Antibody (MSA/953)

• ACTA
• ACTC
• actin, alpha 2, smooth muscle, aorta
• actin, alpha cardiac muscle 1
• actin, alpha skeletal muscle
• actin, aortic smooth muscle
• ACTSA
• alpha-cardiac actin
• ASD5
• ASMA
• Cell growth-inhibiting gene 46 protein
• CFTD
• CFTD1
• CFTDM
• CMD1R
• CMH11
• MPFD
• NEM1
• NEM2
• NEM3
• nemaline myopathy type 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin (Muscle Specific) Antibody (NBP2-45274)(1)

Publication using NBP2-45274

• Miyasaka Y, Murakami K, Ito K et al. Condensed desmin and actin cytoskeletal communication in lipid droplets Cytoskeleton (Hoboken) 2019 Oct 18 [PMID: 31626391] (WB)

Bioinformatics

• Gene Symbol: ACTA1
• Uniprot:
  • P68032()
  • P68032()
  • P62736(Human)
  • P68133(Human)