Actin (Muscle Specific) Antibody (MSA/953)

Images

 
Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Formalin-fixed paraffin-embedded rat stomach stained with muscle specific actin monoclonal antibody (MSA/953)
Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control ...read more
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Flow Cytometry: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control ...read more
Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Rt, RbSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-P, ICC/IF
Clone
MSA/953
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Actin (Muscle Specific) Antibody (MSA/953) Summary

Immunogen
Synthetic peptide common among human muscle actins
Localization
Cytoplasmic
Marker
Muscle Cell Marker
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.AIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Flow (Intracellular)
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Publications
Read Publication using NBP2-45274.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Actin (Muscle Specific) Antibody (MSA/953)

  • ACTA
  • ACTC
  • actin, alpha 2, smooth muscle, aorta
  • actin, alpha cardiac muscle 1
  • actin, alpha skeletal muscle
  • actin, aortic smooth muscle
  • ACTSA
  • alpha-cardiac actin
  • ASD5
  • ASMA
  • Cell growth-inhibiting gene 46 protein
  • CFTD
  • CFTD1
  • CFTDM
  • CMD1R
  • CMH11
  • MPFD
  • NEM1
  • NEM2
  • NEM3
  • nemaline myopathy type 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin (Muscle Specific) Antibody (NBP2-45274)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Actin (Muscle Specific) Antibody (NBP2-45274) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47662AF350
Alexa Fluor 405 NBP2-47662AF405
Alexa Fluor 488 NBP2-47662AF488
Alexa Fluor 532 NBP2-47662AF532
Alexa Fluor 594 NBP2-47662AF594
Alexa Fluor 647 NBP2-47662AF647
Alexa Fluor 700 NBP2-47662AF700
Alexa Fluor 750 NBP2-47662AF750
Allophycocyanin NBP2-47662APC
Allophycocyanin/Cy7 NBP2-47662APCCY7
Biotin NBP2-47662B
DyLight 350 NBP2-47662UV
DyLight 405 NBP2-47662V
DyLight 488 NBP2-47662G
DyLight 550 NBP2-47662R
DyLight 594 NBP2-47662DL594
DyLight 650 NBP2-47662C
DyLight 680 NBP2-47662FR
DyLight 755 NBP2-47662IR
FITC NBP2-47662F
HRP NBP2-47662H
Janelia Fluor 549 NBP2-47662JF549
Janelia Fluor 646 NBP2-47662JF646
PE NBP2-47662PE
PE/Atto594 NBP2-47662PEATT594
PE/Cy5.5 NBP2-47662PECY55
PE/Cy7 NBP2-47662PECY7
PerCP NBP2-47662PCP

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Bioinformatics

Gene Symbol ACTA1
Uniprot