Actin Antibody (mAbGEa) [DyLight 405]


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Product Details

Reactivity Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Actin Antibody (mAbGEa) [DyLight 405] Summary

Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46]
Cytoplasm > cytoskeleton
Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12.
IgM Kappa
IgM purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
IgM purified


  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Yeast, Xenopus laevis, Drosophila, Zebrafish, Human, Sheep, Mouse, Rat, Bovine, Rabbit, Arabidopsis, Protozoa and Fungi. Chinese hamster data from customer review.Sordaria macrospora reactivity reported in scientific literature (PMID: 24720701)


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Actin Antibody (mAbGEa) [DyLight 405]

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • Alpha-actin-1
  • ASMA
  • CFTD
  • MPFD
  • NEM1
  • NEM2
  • NEM3


Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Antibody (NB100-74340V). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-74340APC
DyLight 350 Labeled NB100-74340UV
DyLight 405LS Labeled NB100-74340V3
DyLight 488 Labeled NB100-74340G
DyLight 680 Labeled NB100-74340FR
DyLight 755 Labeled NB100-74340IR
FITC Labeled NB100-74340F
PE Labeled NB100-74340PE
PerCP Labeled NB100-74340PCP

Additional Actin Antibody (mAbGEa) [DyLight 405] Products

Actin NB100-74340V

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Blogs on Actin. Showing 1-10 of 13 blog posts - Show all blog posts.

Alpha-actin/ACTA1 - A skeletal muscle isoform mutated in various myopathies
Actin is an abundant cytoskeletal protein involved in a variety of cellular processes such as cell motility, cell division, and muscle contraction. Actin monomers assemble into filaments and can provide a track for transport of cargo by the molecul...  Read full blog post.

Alpha-Adducin - Assembling the cytoskeleton meshwork that underlies the plasma membrane
The structure and organization of the plasma membrane is maintained by an underlying network of cytoskeletal proteins including actin and spectrin. Adducin, a member of this protein network, binds to bundles and caps actin filaments and links them...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Actin Dynamics and Endocytic Trafficking
Actin is a ubiquitous and an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes. It is abundant protein whose monomers polymerize into polarized actin filaments, within epithelial cells. Filamentous acti...  Read full blog post.

Beta Actin is a Key Player in Embryonic Development and Cell Motility
Actins are an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes, including cell migration, division, and regulation of gene expression. These functions are attributed to the ability of actin to form fil...  Read full blog post.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Using Myosin Antibodies for Tumor Identification
Myosin is a super family of actin based molecular motors that hydrolyze ATP and generate physical force to move specific molecules inside the cell. This super family, divided into at least twenty four classes based on head domain sequence similarity a...  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol ACTA1

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