Actin Antibody (5C5)

Immunohistochemistry-Paraffin: Actin Antibody (5C5) [NB120-7799] - Formalin fixed paraffin embedded human skeletal muscle stained with Actin Sarcomeric antibody (NB120-7799).

Product Discontinued

Actin Antibody (5C5) Summary

• Immunogen: BALB/C mice were injected with purified rabbit striated muscle actin.
• Localization: Cytoplasmic
• Specificity: Monoclonal anti-ALPHA-sarcomeric actin has been used as a marker for rhabdomyosarcoma. This reacts with a-skeletal and a-cardiac muscle actins. It does not react with smooth muscle tissue.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation inbuffered formalin can destroy the epitope. It is recommended that this product be used on frozen tissue sectionsor specimens. The optimal conditions should be determined by the individual laboratory. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26567220)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26567220). Cross-reacts with sheep, bovine, guinea pig, rat, frog, snake and carp. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Ascites

Alternate Names for Actin Antibody (5C5)

• ACTA
• Actin
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Antibody (NB120-7799)(1)

Publication using NB120-7799

• Wei W, Zhang WY, Bai JB et al. The NF-kB modulated miR-195/497 inhibit myoblast proliferation by targeting Igf1r/Insr and cyclin genes. J. Cell. Sci. 2015-11-13 [PMID: 26567220] (ICC/IF, Mouse)
  
  Details:
  Actin Alpha 1 Skeletal Muscle/ACTA1 antibody used for ICC/IF on C2C12 mouse myoblast cells that were differentiated for 0 and 4 days (Fig 1).

FAQs for Actin Antibody (NB120-7799). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
   • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340 . This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Bioinformatics

• Gene Symbol: ACTA1
• Entrez:
  • 58(Human)
  • 70(Human)
  • 29437(Rat)