Mouse ACTH ELISA Kit (Colorimetric)

ELISA: Mouse ACTH ELISA Kit (Colorimetric) [NBP3-14759] - Samples were spiked with high concentrations of Mouse ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse ACTH ELISA Kit (Colorimetric) [NBP3-14759] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: serum, plasma and other biological fluids
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38pg/mL

Mouse ACTH ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38pg/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 6.60
• Intra-Assay: %CV < 5.94
• Spike Recovery: 88 - 104%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: POMC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse ACTH ELISA Kit (Colorimetric)

• ACTH
• adrenocorticotropic hormone
• Adrenocorticotropin
• alpha-melanocyte-stimulating hormone
• alpha-MSH
• beta-endorphin
• beta-LPH
• beta-melanocyte-stimulating hormone
• beta-MSH
• CLIP
• Corticotropin
• corticotropin-like intermediary peptide
• corticotropin-lipotropin
• gamma-LPH
• gamma-MSH
• lipotropin beta
• lipotropin gamma
• LPH
• melanotropin alpha
• melanotropin beta
• melanotropin gamma
• met-enkephalin
• MSH
• NPP
• POC
• pro-ACTH-endorphin
• proopiomelanocortin preproprotein
• proopiomelanocortin
• pro-opiomelanocortin

Background

ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for ACTH ELISA Kit (NBP3-14759)(2)

Publications using NBP3-14759

• Huifei Sophia Zheng, Yuan Kang, Qiongxia Lyu, Kristina Junghans, Courtney Cleary, Olivia Reid, Greer Cauthen, Karly Laprocina, Chen-Che Jeff Huang, Jacques J. Tremblay DHCR24, a Key Enzyme of Cholesterol Synthesis, Serves as a Marker Gene of the Mouse Adrenal Gland Inner Cortex International Journal of Molecular Sciences 2023-01-04 [PMID: 36674444] (ELISA)
• Cui J, Huang Z, Xie Y et al. Gut microbiota mediated inflammation, neuroendocrine and neurotrophic functions involved in the antidepressant-like effects of diosgenin in chronic restraint stress Journal of Affective Disorders 2022-10-01 [PMID: 36349650] (ELISA)

FAQs for ACTH ELISA Kit (NBP3-14759). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: POMC