Human ACTH ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0.5-165 pg/ml |
| Sensitivity |
0.46 pg/ml |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
Mean 6.0% |
| Intra-Assay |
mean 3.6% |
| Spike Recovery |
Plasma - 101.9% |
| Sample Volume |
200 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
POMC |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Antibody
- Buffer
- Conjugate
- Microtiter Plate
- Standard
- Stop Solution
- Substrate
- Wash Buffer Concentrate
|
Alternate Names for Human ACTH ELISA Kit (Colorimetric)
Background
Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for ACTH ELISA Kit (NBP2-61287). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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