ACTH Antibody (57) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
57
Clonality
Monoclonal
Host
Mouse

ACTH Antibody (57) [DyLight 680] Summary

Immunogen
N-terminal fragment of human ACTH conjugated to KLH
Localization
Cytoplasm and Secreted
Marker
Pituitary Marker
Specificity
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).AIt also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POMC
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 680
Format
DyLight 680 (A=692, E=712)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (No special pretreatment is required for staining of formalin-fixed tissues)

Reactivity Notes

Expected to show broad species reactivity.

Alternate Names for ACTH Antibody (57) [DyLight 680]

  • ACTH
  • adrenocorticotropic hormone
  • Adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • Corticotropin
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACTH Antibody (NBP2-34512FR) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol POMC
Entrez

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