ACTH Antibody (57)

Product Discontinued

ACTH Antibody (57) Summary

• Immunogen: ACTH-KLH Conjugate
• Localization: Secreted
• Specificity: Specific for Synacthen (aa 1-17) Reacts with ACTH (a.a. 1-17) and has no cross- reaction with CLIP (ACTH 17-39). Reacts <1% with insulin, KLH and BSA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POMC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen.
• Control:

  POMC Overexpression Lysate

Reactivity Notes

Cross-reacts with rat N-terminal ACTH.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ACTH Antibody (57)

• ACTH
• adrenocorticotropic hormone
• Adrenocorticotropin
• alpha-melanocyte-stimulating hormone
• alpha-MSH
• beta-endorphin
• beta-LPH
• beta-melanocyte-stimulating hormone
• beta-MSH
• CLIP
• Corticotropin
• corticotropin-like intermediary peptide
• corticotropin-lipotropin
• gamma-LPH
• gamma-MSH
• lipotropin beta
• lipotropin gamma
• LPH
• melanotropin alpha
• melanotropin beta
• melanotropin gamma
• met-enkephalin
• MSH
• NPP
• POC
• pro-ACTH-endorphin
• proopiomelanocortin preproprotein
• proopiomelanocortin
• pro-opiomelanocortin

Background

ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACTH Antibody (NB600-1367)(1)

Publication using NB600-1367

• Kiilerich P, Servili A, Peron S et al. Regulation of the corticosteroid signalling system in rainbow trout HPI axis during confinement stress Gen. Comp. Endocrinol. 2017-08-21 [PMID: 28837788]

Bioinformatics

• Gene Symbol: POMC
• Entrez:
  • 5443(Human)
  • 24664(Rat)
• Uniprot:
  • P01189()